![]() ![]() The testing procedure was designed to evaluate both coarse particles of 5 µm and fine particles from 0.1 µm to 1.0 μm, as established in previous studies. Particle counting was performed using an Aerotek Portable Particle Counter model 9110 (TSI) with a modified flange setup. Representation of the decontamination workflow. ![]() Generic kraft paper lunch bags and autoclavable pouches (Crosstex) were used to contain the FFRs and the masks during the decontamination process ( Figure 1).įigure 1. The decontamination cycles were validated using Comply SteriGage Steam Chemical Integrators (3M) and ATTEST Biological Indicator (3M) in the Super Rapid 5 Steam Plus Challenge Packs (3M). 11, 12 These decontamination cycle parameters were selected not only to control the contamination with SARS-CoV-2 but also to account for potential contamination of the PPE by flora obtained from the clinical environment and/or the end-user. Four autoclave cycles were examined in this study: 115☌ for 1 hour, 11, 12 121.1☌ for 30 minutes, 130☌ for 2 minutes, and 130☌ for 4 minutes. The autoclave cycles were programmed to have 3 prevacuum stages at 1.5 pounds per square inch absolute (psia) each, a sterilization process at the trial exposure temperature and time, and then 10 minutes of drying time. Several cycle strategies were tested, ranging from low temperatures for extended periods of time to high temperatures for short periods.Ī moist heat autoclave was used for this study. Additionally, this method allows for rapid deployment and high throughput decontamination of FFRs. This decontamination capability is available at many hospitals, outpatient clinics, and laboratories where it can be used with standardized training. In this study, we set out to test moist heat autoclave decontamination on N95 FFRs. Some of these methods, although effective, are labor-intensive and require specialized equipment and expertise to perform. 2, 4 - 10 These studies found that multiple decontamination methods can be used on N95 FFRs with varying effects on the functionality and integrity of the FFRs. Several studies have previously been conducted to study the effects of various decontamination methods on PPE, with a focus on FFRs. 1 - 3 Unfortunately, many countries have been unable to meet this demand and are forced to reuse PPE that was initially intended for single use. ![]() ![]() Due to the emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in late 2019 and the subsequent pandemic that it caused, a global increase in demand for PPE has occurred. Medical grade filtering facepiece respirators (FFRs) are an essential form of personal protective equipment (PPE) that help to limit an individual’s exposure to pathogens transmitted by droplets and/or aerosols. ![]()
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